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KMID : 0882420060710020189
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Korean Journal of Medicine
2006 Volume.71 No. 2 p.189 ~ p.197
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Evaluation of repetitive sequence-based genomic fingerprinting for molecular classification and identification of vibrio species
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Kim Gyu-Won
Chung Hun-Jong
Park Chul-Min
Kim Ki-Jeong
Kim Won-Yong
Chung Sang-In
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Abstract
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Background: The aims of this study were to compare the suitability of repetitive-PCR genomic fingerprinting procedures to investigate genetic relatedness of the genus Vibrio and its applicability for the molecular identification of Vibrio vulnificus.
Methods: Forty-eight Vibrio strains were included for this study. REP-, ERIC-, BOX- and SERE-PCR were compared with 13 members of the genus Vibrio.
Results: REP-, BOX- and SERE-PCR showed V. vulnificus strains could not be separated well from other Vibrio species. However, approximately 320 bp of highly discriminatory specific fragments was recovered from V. vulnificus strains by ERIC-PCR.
Conclusion: ERIC-PCR could be used as rapid classification and identification methods of V. vulnificus from other members of the genus Vibrio.
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KEYWORD
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Vibrio species, Vibrio vulnificus, rep-PCR genomic fingerprinting
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